RESISTANCE STATUS AND MECHANISM OF Aedes aegypti AGAINST THREE CLASSES OF INSECTICIDES IN SALATIGA CITY, INDONESIA

Authors

  • Muhammad Choirul Hidajat National Research and Innovation Agency
  • Riyani Setyaningsih Institute for Vector and Reservoir Control Research and Development, Ministry of Health, Indonesia
  • Yusnita Mirna Anggraeni Research Organization for Health, National Research and Innovation Agency, Indonesia
  • Wiwik Trapsilowati
  • Triwibowo Ambar Garjito Research Organization for Health, National Research and Innovation Agency, Indonesia

DOI:

https://doi.org/10.17501/24246735.2022.7108

Keywords:

Resistance, Aedes aegypti, Salatiga, Insecticide

Abstract

Insecticide resistance is a major problem in dengue vector control in Indonesia. Salatiga
City, one of Central Java's dengue-endemic areas, has been using insecticide for vector
control for decades. To select the insecticide used, it is necessary to understand the status
and mechanism of resistance to various insecticides. This study aimed to determine the
status and mechanism of Aedes aegypti resistance in Salatiga City. This study used a
descriptive method. Larvae were collected from 8 endemic villages in Salatiga City. The
larvae were then reared to produce adult mosquitoes. A resistance test was conducted
using the WHO-Impregnated paper method on F1 mosquitoes. The insecticides used for
the test were malathion 5%, phenytotrione 1% (Organophosphate); Bendiocarb 0.1%,
Propoxur 0.1% (Carbamate); Lambda Cyhalothrin 0.03%, Deltamethrin 0.05%,
Permethrin 0.25%, and Cypermethrin 0.05.% (Pyretrhoid). To determine the resistance
mechanism of the target site mutation, sequencing of the Domain II VGSC and ACE1
genes was carried out. The test results showed that Ae. aegypti from 8 locations were
resistant to all insecticides. The highest mortality after 24 hours observation was on
organophosphate group. Molecular tests showed that Single Nucleotide Polymorphism had
occurred in the S989P and V1016G alleles of the VGSC gene, indicating molecular
resistance to Pyretrhoid insecticides. The ACE1 gene sequences showed no mutations in
the G119S allele, indicating the possibility of metabolic resistance to organophosphates
and carbamates. In case of a dengue outbreak, it is recommended to use organophosphate
because it has the highest percentage of mosquito mortality, and only metabolic resistance
occurs without target site mutations.

Downloads

Download data is not yet available.

References

Brengues, C., N.J.Hawkes, Chandre, F., L.Mccarroll, Duchon, S., Guillet, P., MANGUIN, S. ., J.C.MORGAN, HEMINGWAYy, J., & Hemingway, J. (2003). Pyrethroid and DDT cross-resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated sodium channel gene. Medical and Veterinary Entomology, 17, 87–94.

Davies, T. G. E., & Williamson, M. S. (2009). Interactions of pyrethroids with voltage gated sodium channel. Bayer CropScience Journal, 62(January), 159–178.

Elyazar, I. R. F., Hay, S. I., & Baird, J. K. (2011). Malaria Distribution, Prevalence, Drug Resistance and Control in Indonesia. Adv Parasitol, 1–107. https://doi.org/10.1016/B978-0-12-385897-9.00002-1.Malaria

Hemingway, J., Hawkes, N. J., Mccarroll, L., & Ranson, H. (2004). The molecular basis of insecticide resistance in mosquitoes. Insect Biocheistry and Molecular Biology, 34, 653–665. https://doi.org/10.1016/j.ibmb.2004.03.018

Hsan, K., Hossain, M. M., Sarwar, M. S., Wilder-Smith, A., & Gozal, D. (2019). Unprecedented rise in dengue outbreaks in Bangladesh. The Lancet Infectious Diseases, 19(12), 1287. https://doi.org/10.1016/S1473-3099(19)30616-4

Islami, S., Puspa, A., Hidayati, N., Wibowo, H., & Syafruddin, D. (2018). The role of Voltage-Gated Sodium Channel ( VGSC ) gene mutations in the resistance of Aedes aegypti L . to pyrethroid permethrin in Palembang and Jakarta, Indonesia. Preprints, March, 1–6. https://doi.org/10.20944/preprints201803.0070.v1

Kusumastuti, N. H. (2014). Use of House Insecticide in Pangandaran Village. Widyariset, 3, 417–424.

Maria de Lourdes da Graça Macoris, Andrighetti, M. T. M., Otrera, V. C. G., Carvalho, L. R. de, Júnior, A. L. C., & Brogdon, W. G. (2007). Association of insecticide use and alteration on Aedes aegypti susceptibility status. Mem Inst Oswaldo Cruz, 102(December), 895–900.

Martins, A. J., Mazzei, R., Andrade, M. De, Gerlinde, J., Linss, B., Peixoto, A. A., & Valle, D. (2009). Voltage-Gated Sodium Channel Polymorphism and Metabolic Resistance in Pyrethroid-Resistant Aedes aegypti from Brazil. Am.J.Trop.Med.Hyg., 81(June 2014), 108–115.

Maula, A. W., Fuad, A., Utarini, A., & Maula, A. W. (2018). Ten-years trend of dengue research in Indonesia and South-east Asian countries : a bibliometric analysis. Global Health Action, 11(1), 1–8. https://doi.org/10.1080/16549716.2018.1504398

Mitchell, S. N., Rigden, D. J., Dowd, A. J., Lu, F., Wilding, C. S., Weetman, D., Dadzie, S., Jenkins, A. M., Regna, K., Boko, P., Djogbenou, L., Muskavitch, M. A. T., Ranson, H., Paine, M. J. I., Mayans, O., & Donnelly, M. J. (2014). Metabolic and Target-Site Mechanisms Combine to Confer Strong DDT Resistance in Anopheles gambiae. PLOS ONE, 9(3), 1–10. https://doi.org/10.1371/journal.pone.0092662

Plernsub, S., Saingamsook, J., Yanola, J., Lumjuan, N., Tippawangkosol, P., Walton, C., & Somboon, P. (2016). Temporal frequency of knockdown resistance mutations, F1534C and V1016G, in Aedes aegypti in Chiang Mai city, Thailand and the impact of the mutations on the efficiency of thermal fogging spray with pyrethroids. Acta Tropica, 162, 125–132. https://doi.org/10.1016/j.actatropica.2016.06.019

Riani, D. A., Wahyuningsih, N. E., & Budiharjo, A. (2017). Hubungan Praktik Penggunaan Insektisida Dengan Kejadian Demam Berdarah Dengue Di Semarang. Jurnal Kesehatan Masyarakat (e-Journal), 5(5), 592–598.

Rueda, L. M. (2004). Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission. In Zootaxa (1st ed., Vol. 589, Issue 1). Magnolia Press. https://doi.org/10.11646/zootaxa.589.1.1

Sayono, S., Hidayati, A. P. N., Fahri, S., Sumanto, D., Dharmana, E., Hadisaputro, S., Asih, P. B. S., & Syafruddin, D. (2016). Distribution of voltage-gated sodium channel (NAV) alleles among the aedes aegypti populations in central Java province and its aociation with resistance to pyrethroid insecticides. PLoS ONE, 11(3), 1–12. https://doi.org/10.1371/journal.pone.0150577

Singhi, S. C. (2018). Severe Dengue: Developing a Universally Applicable Simple Prediction Model. Indian Journal of Pediatrics, 85(6), 413–414. https://doi.org/10.1007/s12098-018-2668-2

Siti-Futri, F. F., Rosilawati, R., Wan, K. L., Cheong, Y. L., Nazni, W. A., & Lee, H. L. (2020). Status of pyrethroid resistance in Aedes (Stegomyia) aegypti (linneaus) from dengue hotspots in Klang valley, Malaysia. Tropical Biomedicine, 37(1).

Widiarti, Heriyanto, B., Boewono, D. T., Widiastuti, U., Mujiono, Lasmiati, & Yuliadi. (2011). The Resistance Map of Dengue Haemorrhargic Fever Vector Aedes aegypti Againts Organophosphates, Carbamates and Pyrethroid Insecticides in Central Java and Yogyakarta Province. Bul. Penelit. Kesehat, 39(4), 176–189.

Wigati, R. A., & Susanti, L. (2012). Hubungan karakteristik pengetahuan, sikap dan perilaku masyarakat dalam menggunakan antinyamuk di Kelurahan Kutowinangun. Buletin Penelitian Kesehatan, 40(3).

Wuliandari, J. R. (n.d.). Frequency of kdr mutations in the voltage-sensitive sodium channel ( VSSC ) gene in Aedes aegypti from Yogyakarta and implications for Wolbachia-infected mosquito trials. 1–34.

Wuliandari, J. R., Lee, S. F., White, V. L., Tantowijoyo, W., Hoffmann, A. A., & Endersby-Harshman, N. M. (2015). Association between three mutations, F1565C, V1023G and S996P, in the voltage-sensitive sodium channel gene and knockdown resistance in aedes aegypti from yogyakarta, Indonesia. Insects, 6(3), 658–685. https://doi.org/10.3390/insects6030658

Yu, F. H., Catterall, W. A., Hodgkin, A., Huxley, A., Hille, B., Catterall, W., Rohl, C., Boeckman, F., Baker, C., Scheuer, T., Catterall, W., Klevit, R., Doyle, D., Cabral, J., Pfuetzner, R., Gulbis, J., Cohen, S., Chait, B., MacKinnon, R., … Vandenberg, J. (2003). Overview of the voltage-gated sodium channel family. Genome Biology, 4(3), 207. https://doi.org/10.1186/gb-2003-4-3-207

Downloads

Published

2022-11-03

How to Cite

Hidajat, M. C., Setyaningsih , R., Anggraeni, Y. M., Trapsilowati, W., & Garjito, T. A. (2022). RESISTANCE STATUS AND MECHANISM OF Aedes aegypti AGAINST THREE CLASSES OF INSECTICIDES IN SALATIGA CITY, INDONESIA. Proceedings of the International Conference on Public Health, 7(1), 83–91. https://doi.org/10.17501/24246735.2022.7108