Effect of Mealworm (Ternebrio molitor) Meal in Practical Diets on Growth Performance, Feed Utilization and Carcass Composition of Climbing Perch Fingerlings (Anabas testudineus)


  • warrapong nalinanon King Mongkut’s Institute of Technology Ladkrabang Prince of Chumphon Campus
  • S Lerdsuwan Program of Animal Science, King Mongkut’s Institute of Technology Ladkrabang, Prince of Chumphon Campus, Thailand
  • D Pisuttharachai Program of Fishery Science and Aquatic Resources, King Mongkut’s Institute of Technology Ladkrabang, Prince of Chumphon Campus, Thailand




Mealworm, growth performance, feed utilization, Anabas testudineus


The Climbing Perch (Anabas testudineus), a tropical freshwater fish, was chosen as a candidate for commercial inland aquaculture. Two separate 12-week feeding trials were conducted to evaluate the use of mealworm, Tenebrio molitor, as an alternative protein source for A. testudineus. In Experiment 1, fingerlings of A. testudineus 8.6 + 0.1 g were acclimated to laboratory conditions for a period of 2 weeks. Five isonitrogenous (30% crude protein) and isoenergetic (4,000 Kcal/Kg) practical diets were formulated. The fish meal component of the diets was progressively substituted at 0, 12.5, 25, 37.5, and 50% with mealworm meal. The diets were fed to triplicate groups of 20 fish twice a day to apparent satiation. Growth performance and feed utilization efficiency of

A. testudineus fed diets with up to 25% replacement of fish meal with worm meal were not significantly different (P>0.05) in FCR, PER and NPU as 1.51±0.09, 2.22±0.14 and 34.06±1.05% respectively compared to fish fed the control diet. Worm meal in the experimental diets of caused a significant (p<0.05) increase in whole body lipid and decrease in moisture at all levels of worm meal incorporation. Treatment 4 (37.5% worm meal) was the highest in whole body lipid as 9.50±0.82 % and treatment 5 (50% worm meal) was the lowest in moisture as 70.45±1.76 %. In Experiment 2, fingerlings of A. testudineus 15.2+0.12 g were acclimated to laboratory conditions for a period of 2 weeks. The nutritive value of mealworms was compared with a commercial catfish pellet. Five dietary treatments consisting of fish fed catfish pellets only, mealworms (25) /catfish pellet (75) combination, mealworms (50) /catfish pellet (50) combination, mealworms (75) /catfish pellet (25) combination and mealworms only were tested. A. testudineus fed mealworms (50) /catfish pellet (50) combination displayed the best in specific growth rate as 2.61±0.10 than A. testudineus fed catfish pellets only


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Abd Rahman Jabir, M.D., Razak, S.A. and Vikineswary, S. (2012). Nutritive potential and utilization of super worm (Zophobas morio) meal in the diet of Nile tilapia (Oreochromis niloticus) juvenile. African Journal of Biotechnology, 11(24), 6592-6598.
Association of Official Analytical Chemists (AOAC). (1997). Official Methods of Analysis, 16th edn (ed. by P. A. Cunniff). AOAC International, Arlington, VA. USA.
Bovera, F., Piccolo, G., Gasco, L., Marono, S., Loponte, R., Vassalotti, G., Mastellone, V., Lombardi, P., Attia, Y.A. and Nizza, A. (2015). Yellow mealworms larvae (Tenebrio molitor, L.) as protein source for broilers: effects on growth performance and blood profiles. Brit. Poultry Sci, 56(5), 569-575.
Ebeling, W. (1975). Urban entomology. Pest of stored food products. Division of Agriculture Sciences, University of California: Berkeley, USA.
Habib, M.A.B., Hasan, M.R., Akand, A.M. and Siddiqua, A. (1994). Evaluation of silkworm pupae meal as a dietary protein source for Clarias batrachus fingerlings. Aquaculture, 124, 61-66.
Hardy, R.W. and Tacon, G.J. (2002). Fish meal: historical uses, production trends and future outlook for supplies. Responsible Marine Aquaculture, 311-325.
Kono, M., Matsui, T. and Shimizu, C. (1987). Effect of chitin, chitosan and celllulose as diet supplements on the growth of cultured fish. Nippon Suisan Gakkaishi, 53, 125-129.
Murai, T. and Andrews, J.W. (1978). Riboflavin requirement of channel catfish fingerlings. J. Nutr, 108(9), 1512-1517.
Nalinanon, W. and Lerdsuwan, S. (2018). Effect of dietary salt (NaCl) supplementation on growth, feed utilization and carcass quality of Nile tilapia (Oreochromis niloticus). The Proceedings of Suratthani Rajabhat University Conference 2018 International Conference on Innovations in Interdisciplinary Research (ICIIR), 179-184.
Nandeesha, M.C., Srikanth, G.K., Varghese, T.J., Keshavanath, P. and Shetty, H.P.C. (1988). Influence of silkworm pupa based diets on growth, organoleptic quality and biochemical composition of catla-rohu hybrid. Aquaculture Research in Asia, 211-221.
Nengas, I., Alexis, M.N. and Davies, S.J. (1999). High inclusion levels of poultry meals and related by products in diets for gilthead sea bream, (Sparus aurata L). Aquaculture, 179, 13-23.
Ng, W.K., Liew, F.L., Ang, L.P. and Wong, K.W.( 2001). Potential of mealworm (Tenebrio molitor) as an alternative protein source in practical diets for African catfish, (Clarias gariepinus). Aquaculture Research, 32 (Suppl. 1), 273-280.
Ovie, S.O. and Ovie, S.I. (2007). The effect of replacing fish meal with 10% groundnut cake in the diets of H. longifilis on its growth, food conversion and survival. J. Appl. Sci. Environ. Manage, 11(3), 87-90.
Plaipetch, P., Kuekaew, J., Tamtin, M. and Choncheunchob, P. (2014). Effects of Low Dietary Fishmeal on Growth, Nitrogen Loading and Some Physical and Chemical Indices of Asian Sea Bass Lates calcarifer (Bloch, 1790). Kasetsart Journal (Nat. Sci.), 48, 72-82.
Shiau, S.Y. and Yu, Y.P. (1999). Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreochromis niloticus x O. aureaus. Aquaculture, 179, 439-446.
Tacon, A.G.J., Stafford, E.A. and Edwards, C.A. (1983). A preliminary investigation of the nutritive value of three terrestrial lumbricid worms for rainbow trout. Aquaculture, 35, 187-199.




How to Cite

nalinanon, warrapong, Lerdsuwan, S., & Pisuttharachai, D. (2021). Effect of Mealworm (Ternebrio molitor) Meal in Practical Diets on Growth Performance, Feed Utilization and Carcass Composition of Climbing Perch Fingerlings (Anabas testudineus). Proceedings International Conference on Fisheries and Aquaculture, 6(1), 29–37. https://doi.org/10.17501/23861282.2020.6104