MICROPROPAGATION OF Dendrocalamus sericeus
DOI:
https://doi.org/10.17501/26827018.2024.9104Keywords:
plant tissue culture, Bamboo, node explant, growth regulators, shoot induction and shoot multiplicationAbstract
Micropropagation of Sang Mon bamboo tissue culture using bamboo node explant can increase the number of plants for rapid utilization. Therefore, this experiment aimed to study the sterilization of node explant and suitable medium for shoot induction and shoot multiplication. The method suitable for disinfecting node explant for bamboo was established. First, the node explant was washed and thoroughly rinsed with soap, then immersed in 70% alcohol for 2 min, in 10% Clorox® for 15 min, in 5% Clorox® for 10 min, (Add 1-2 drops of Tween20 to Clorox®) and in fungicide (Fosetyl-aluminium) at a concentration of 1.5 g L-1 for 1 hr. The bamboo node was washed three times of autoclaved distilled water. It was found that the leas microbial contamination was 25.89±6.22% and the number of shoots was 1.35±0.24 shoots/node. This disinfection method was applied before shoot induction and shoot multiplication. For shoot induction, nodes were cultured on solid MS medium supplemented with 0, 2, 4 and 6 mg L-1 BA, with 2, 4 and 6 mg L-1 BA and 1 mg L-1 KIN and with 2, 4 and 6 mg L-1 BA and 1 mg L-1 NAA for 4 weeks. The results showed that the explants cultured on MS medium supplemented with 4 mg L-1 BA had a maximum induced shoot at 67.53±7.80%, an average of shoot numbers at 4.03±0.23 shoots/node and shoot length of 3.52±0.44 cm. Then, three shoots per clump were sub-cultured onto solid medium or liquid MS medium supplemented with 0, 2, 3 and 4 mg L-1 BA for shoot multiplication. After cultured for two weeks the results showed that medium-stage and BA concentrations were not significantly different on shoot multiplications and shoot length. Hence, this protocol can be used for micropropagation of D. sericeus and a database for the tissue culture of other bamboo species.
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