THE EFFECT OF BIO-STIMULANT APPLICATION METHODS ON THE GROWTH AND PHYTOCHEMICAL CONTENT OF CHILLI SEEDLINGS
DOI:
https://doi.org/10.17501/26827018.2023.8105Keywords:
bio-stimulant, nano-encapsulation, bacteria, moringa, drenching, foliarAbstract
Bio-stimulants have gained vast recognition in recent years as vigorous research has been conducted to improve crop growth, yield, and quality. However, bio-stimulant utilization in soilless systems has been limited due to their rapid degradation and short lifespan. New formulations are essential to extend the longevity of bioactive compounds in the target site. This paper highlights the effect of different application methods, such as drenching soilless medium and foliar application, on the chlorophyll, total phenolic, flavonoid, and carotenoid contents in chilli leaf. A completely randomized block design (RCBD) consisting of five treatments and three replications with ten plants per replication was adopted in this study. Moringa leaf extract (MLE) was prepared in a nano-emulsion form while bacteria were incorporated in nano-gel. Data were analyzed using the SPSS software (IBM Statistics, version 28, USA). Foliar application of MLE nano-emulsion significantly increased the chlorophyll content (35.958±7.19 mg g-1) in chilli leaf than the drenching method (26.545±0.5 mg g-1) and non-encapsulated MLE foliar treatment (12.233±2.33 mg g-1). In addition, carotenoid (2.138±1.68 mg g-1) and phenolic contents (1.090±0.17 mg gallic acid equivalent g-1) in chilli leaf were higher when nano-emulsion of MLE used as foliar application than non-encapsulated MLE (1.725±1.08 mg g-1 of carotenoid and 0.993±0.16 mg gallic acid equivalent g-1 of phenolic content). Compared to other treatments, the seedlings treated with encapsulated bacteria and nano-emulsion of MLE showed a significant increase in plant height. It was found that MLE nano-emulsion improved the photosynthetic activities of chilli plants significantly by increasing the chlorophyll and carotenoid contents when used as a foliar application. Nano-encapsulation technique protects the active ingredients in MLE and increases the absorption due to their nano-size and large surface area.
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