Drought and Biostimulant Treatments Affected Organic Acid Content of Tomato Seedlings


  • E Yildirim Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey
  • M Ekinci Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey
  • M Yüce Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey
  • M Turan Department of Agricultural Trade and Management, Yeditepe University, Ataşehir, Istanbul, Turkey
  • S Ors Department of Agricultural Structure and Irrigation, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey




bacteria, drought, organic acid, stress, tomato


Water stress causes significant problems in plant growth and development in arid and semi-arid regions in the world. Water stress symptoms especially appears during the seedling period of plants in many crops. However, the tolerance of plants to water stress can be increased with some exogenous biostimulant applications. The present study investigated the effect of exogenous biostimulant application on organic acid content of tomato seedlings under water stresses conditions. The study was conducted as pot experiment under controlled greenhouse conditions.  Drought stress treatments was applied in two different levels; full irrigation (100%) and 50% of the field capacity in the study. 1% Zn, Bacillus subtilis, Bacillus megaterium, Azosprillum and Bacillus amyloliquefaciens (1x109cfu/ml) mixture were used as a biostimulant treatment. The biostimulant solutions were prepared at a ratio of 1/10 and 1/5 and given to the tomato seedlings three times with one-week intervals as root drench. The effects of water stress and the solutions on organic acid content of tomato seedlings were determined in the study. The results were differed depending on the organic acid type under water stress and non-stress conditions. However, depending on the application doses; the negative effect of lower irrigation level on the organic acid content was alleviated. The results of the biostimulant application doses found statistically significant. In most of the organic acids, the application dose makes massive differences on the content of the organic acids. As a result, it is thought that the effect of lower irrigation level on tomato seedlings in terms of organic acid content can be improved by exogenous biostimulant applications.


Backer, R., Rokem, J. S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S. & Smith, D. L. (2018). Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science, 1473.

Bucio, J.L., Jacobo, M.F.N., Rodrı́guez, V.R. & Estrella, L. H. (2000). Organic acid metabolism in plants: from adaptive physiology to transgenic varieties for cultivation in extreme soils. Plant Science, 160(1), 1-13.

Farooq, M., Hussain, M., Wahid, A. & Siddique, K.H.M. (2012). Drought Stress in Plants: An Overview. In: Aroca, R. (eds) Plant Responses to Drought Stress. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32653-0_1

Greene, J.G., Porter, R.H., Eller, R.V. &Greenamyre, J.T. (1993). Inhibition of succinate dehydrogenase by malonic acid produces an ‘excitotoxic’ lesion in rat striatum. Neurochemistry, 61, 1151-1154.

Gupta, A., Dixit, S.K. & Senthil-Kumar, M. (2016). Drought Stress Predominantly Endures Arabidopsis thaliana to Pseudomonas syringae Infection. Front Plant Science, 7, 808.

Kiran, S., Kusvuran, S., Ates, C., Ellialtıoğlu, S.S. (2018). The changes of fruit quality parameters at using of different eggplant rootstock/scion combinations which growing under salt and drought stress. Derim, 2018/35(2):111-120 doi: 10.16882/derim.2018.427095

Marasco, R., Rolli, E., Ettoumi, B., Vigani, G., Mapelli, F., Borin, S., Abou-Hadid, A.F., El-Behairy, U.A., Sorlini, C., Cherif, A., Zocchi, G. &Daffonchio, D. (2012). A drought resistance promoting microbiome is selected by root system under desert farming. PLoS ONE 7, e48479.

Panchal, P., Miller, A. J., & Giri, J. (2021). Organic acids: versatile stress-response roles in plants. Journal of Experimental Botany, 72(11), 4038-4052.

Rivas-Ubach, A., Sardans, J., Perez-Trujillo, M., Estiarte, M. &Penuelasa, J. (2012). Strong relationship between elemental stoichiometry and metabolome in plants. Proceedings of the National Academy of Sciences, 109(11), 4181–4186.

Ruzzi, M. & Aroca, R. (2015). Plant growth-promoting rhizobacteria act as biostimulants in horticulture. Scientia Horticulturae, 196, 124-134.

Salisbury, F.B. & Ross, C.W. (1997). Plant Physiology, 4 th. Edition, Belmont, California, USA: Wadsworth Publishing Company.

Siddiqui, S. N., Umar, S. & Iqbal, M. (2015). Zinc-induced modulation of some biochemical parameters in a high- and a low-zinc-accumulating genotype of Cicer arietinum L. grown under Zn-deficient condition. Protoplasma, 252, 1335-1345.

Song, F., Han, X., Zhu, X. &Herbert, S.J. (2012). Response to water stress of soil enzymes and root exudates from drought and non-drought tolerant corn hybrids at different growth stages. Canadian Journal of Soil Science, 92, 501-507.

SPSS, 2010. SPSS Inc. 18.0 Base User’s Guide. Chicago (IL): Prentice Hall, USA, 2010.

Webb, M.A., Cavaletto, J.M., Carpita, N.C., Lopez, L.E. & Arnott, H.J. (1995). The intravacuolar organic matrix associated with calcium oxalate crystals in leaves of Vitis. Plant Journal, 7, 633- 648.

Zolman, B.K., Martinez, N., Millius, A., Adham, A.R. & Bartel, B. (2008). Identification and characterization of Arabidopsis indole-3-butyric acid response mutants defective in novel peroxisomal enzymes. Genetics, 180, 237–251.




How to Cite

Yildirim , E., Ekinci , M., Yüce, M., Turan , M., & Ors, S. (2022). Drought and Biostimulant Treatments Affected Organic Acid Content of Tomato Seedlings. Proceedings of the International Conference on Agriculture, 7(1), 21–28. https://doi.org/10.17501/26827018.2022.7103