Effects of Biostimulant Treatments on Amino Acid Content of Tomato Seedling under Water Deficit
DOI:
https://doi.org/10.17501/26827018.2022.7101Keywords:
amino acid content, drought stress, tomato seedling, bio-stimulantAbstract
In this study, the effect of biostimulant treatment against water stress in tomato seedling period was investigated. In the study conducted in the greenhouse conditions, water stress was applied in two different levels; full irrigation (100% of the field capacity) and 50% of the field capacity. The product used as a biostimulant has 1%Zn, Bacillus subtilis, Bacillus megaterium, Azosprillum and Bacillus amyloliquefaciens (1x109 cfu/ml) content. The solutions prepared at a ratio of 1/10 and 1/5 and were given to the plants three times with one-week intervals. The effects of water stress and biostimulant treatments on amino acid content in tomato seedlings were investigated. As a result of this study, some of the amino acid content in the plant leaf with has increased and some have decreased under different water availability conditions according to the amino acid type. However, depending on the application doses, it was seen that the negative effect of water stress on the amino acid content was alleviated. As a result of the current study, the effect of applications in reducing the damage caused by water stress on tomato seedlings may also be important in terms of amino acid content.
Downloads
References
Ahluwalia, O., Singh, P. C. & Bhatia, R. (2021). A review on drought stress in plants: Implications, mitigation and the role of plant growth promoting rhizobacteria. Resources, Environment and Sustainability, 5, 100032.
Aristoy, M. C. &Toldra, F. (1991). Deproteinization techniques for HPLC amino acid analy-sis in fresh pork muscle and dry-cured ham. Journal of Agricultural and Food Chemistry, 39, 1792-1795.
Antoine, F. R., Wei, C. I., Littell, R. C. & Marshall, M. R. (1999). HPLC method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn derivatization. Journal of Agricultural and Food Chemistry, 47, 5100-5107.
Backer, R., Rokem, J. S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S. & Smith, D. L. (2018). Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science, 1473.
Farooq, M., Hussain, M., Wahid, A. & Siddique, K. H. M. (2012). Drought stress in plants: an overview. Plant Responses to Drought Stress, 1-33.
Haghighi, T. M., Saharkhiz, M. J., Kavoosi, G. & Jowkar, A., 2022. Monitoring amino acid profile and protein quality of Licorice (Glycyrrhiza glabra L.) under drought stress, silicon nutrition and mycorrhiza inoculation. Scientia Horticulturae, 295, 110808.
Henderson, J. W., Ricker, R. D., Bidlingmeyer, B. A. & Woodward, C. (1999). Amino acid analysis using Zorbax Eclipse-AAA Columns and the Agilent 1200 HPLC.
Hildebrandt, T. M., Nesi, A. N., Araújo, W. L. & Braun, H. P. (2015). Amino acid catabolism in plants. Molecular Plant, 8(11), 1563-1579.
Lanzinger, A., Frank, T., Reichenberger, G., Herz, M. & Engel, K. H. (2015). Metabolite profiling of barley grain subjected to induced drought stress: responses of free amino acids in differently adapted cultivars. Journal of Agricultural and Food Chemistry, 63(16), 4252-4261.
Lephatsi, M., Nephali, L., Meyer, V., Piater, L. A., Buthelezi, N., Dubery, I. A., Opperman, H., Brand, M., Huyser, J. & Tugizimana, F. (2022). Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants. Scientific Reports, 12(1), 1-18.
Marasco, R., Rolli, E., Ettoumi, B., Vigani, G., Mapelli, F., Borin, S., Abou-Hadid, A.F., El-Behairy, U.A., Sorlini, C., Cherif, A., Zocchi, G. & Daffonchio, D. (2012). A drought resistance promoting microbiome is selected by root system under desert farming. PLoS ONE 7: e48479.
Moe, L. A. (2013). Amino acids in the rhizosphere: from plants to microbes. American Journal of Botany, 100(9), 1692-1705.
Nephali, L., Moodley, V., Piater, L., Steenkamp, P., Buthelezi, N., Dubery, I., Burgess, K., Huyser, J. & Tugizimana, F. (2021). A metabolomic landscape of maize plants treated with a microbial biostimulant under well-watered and drought conditions. Frontiers in Plant Science, 12, 676632.
Reggiani, R., Nebuloni, M., Mattana, M. & Brambilla, I. (2000). Anaerobic accumulation of amino acids in rice roots: role of the glutamine synthetase/glutamate synthase cycle. Amino Acids, 18, 207– 217.
Ruzzi, M. & Aroca, R. (2015). Plant growth-promoting rhizobacteria act as biostimulants in horticulture. Scientia Horticulturae, 196, 124-134.
Salehi-Lisar, S. Y. & Bakhshayeshan-Agdam, H. (2016). Drought stress in plants: causes, consequences, and tolerance. In Drought Stress Tolerance in Plants, Vol 1 (pp. 1-16). Springer, Cham.
Sircelj, H., Batic, F. & Stampar, F. (1999). Effects of drought stress on pigment, ascorbic acid and free amino acids content in leaves of two apple tree cultivars. Phyton-Horn, 39(3), 97-100.
Van Oosten, M. J., Pepe, O., De Pascale, S., Silletti, S. & Maggio, A. (2017). The role of biostimulants and bioeffectors as alleviators of abiotic stress in crop plants. Chemical and Biological Technologies in Agriculture, 4(1), 1-12.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Proceedings of the International Conference on Agriculture
This work is licensed under a Creative Commons Attribution 4.0 International License.
