ECO-FRIENDLY MANAGEMENT COMMON LAB CONTAMINANT Trichoderma spp IN OYSTER MUSHROOM PRODUCTION USING AGROBASED INDUSTRY’S BY-PRODUCTS

  • K Pakeerthan
Keywords: Pleurotus ostreatus, eco-friendly, plant extract, substrate, coffee, paddy straw

Abstract

An abundant supply of low-cost substrate and management of green mold disease-causing fungus Trichoderma are the major hurdles in successful mushroom production. This study aimed to identify the best Agro-based industry’s by-products as a substitute for oyster mushroom production (Pleurotus ostreatus) while managing fungal contaminants eco-friendly. Two sets of In-Vitro [containing 20% extracts, from agro-based
industries, such as coffee waste powder, tea dust and Mahua oil cake] and In-Vivo experiments [four substrates such as paddy straw, wood sawdust, paddy husk and banana leaves were incorporated with coffee powder, tea dust and Mahua oil cake] were prepared separately. All the experiments were conducted using a complete randomized design with three replicates. The In-Vitro data [mycelial growth and sporulation of both fungi], In-Vivo data [mycelial mushroom run, pinhead formation and yield] were subjected to ANOVA and DMRT mean separation using SAS 9.1 statistical package at P <0.05. In-Vitro results showed that the Trichoderma mycelial growth was significantly minimum in Mahua (2.5 cM) and coffee (3.6 cM) in comparison to control, whereas, with decreasing concentration of coffee, tea, and Mahua extract P. ostreatus showed enhanced growth. Trichoderma sporulation had significantly affected coffee treatment, and even not sporulate in Mahua treated plants. The In-Vivo experiment proved that spawn run was consistent and significant among the treatments when mixed tea (20 days) and coffee (21 days), respectively, at P <0.05. Treatment wise coffee treated spawn bags took an average of 32.5 days, whereas, in tea-treated substrates, it was more than 36 days to form pinhead. Mahua treated trials showed poor spawn run in all substrates, longer days of pinhead formation, and lower yield. In contrast, the paddy straw + coffee treatment produced a significantly highest yield of 200.67g. When sawdust was the substrate, the addition of tea showed a significantly higher yield of 185.00g than coffee (145.00g). In conclusion, coffee and tea extracts have a significant effect on yield with paddy straw and sawdust while minimizing the growth of Trichoderma.

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References

Choi, I., Hong, S., & Yadav, M. C. (2018). Molecular and Morphological Characterization of Green

Mold, Trichoderma spp. isolated from Oyster Mushrooms, Mycobiology, 31:2, 74-80, DOI:

https://doi.org/10.4489/MYCO.2003.31.2.074

Dubey, D., Dhakal, B., Dhami, K., Sapkota, P., Rana, M., Poudel, N. S., & Aryal, L. (2019).

Comparative study on effect of different substrates on yield performance of oyster mushroom. Global

Journal of Biology, Agriculture & Health Sciences, 7, 1–7.

Fan, L., Soccol, A. T., Pandey, A., Porto, L., & Vandenberghe, D. S. (2006). Effect of caffeine and

Tannins on cultivation and fructification of Pleurotus on coffee husks. Brazilian Journal of

Microbiology, 37, 420–424. https://doi.org/10.1590/S1517-83822006000400003

FAO (2004). Wild edible fungi, a global overview of their use and importance to people, by E. Boa,

Non-Wood Forest Products No. 17, Rome (http://www.fao.org/3/i0522e/i0522e.pdf)

Proceedings of the 5th International Conference on Climate Change, Vol. 5, Issue 1, 2021, pp. 65-71

Jayalal, R. G. U., & Adikaram, N. K. B. (2007). Influence of Trichoderma harzianum Metobolites on

the development of green mould disease in the Oyster mushroom. Ceylon Journal of Science (Bio

Science), 36(1), 53–60

Martínez-Carrera D., Aguilar A., Martínez W., Bonilla M., Morales P., Sobal M. (2000) Commercial

Production and Marketing of Edible Mushrooms Cultivated on Coffee Pulp in Mexico. In: Sera T.,

Soccol C.R., Pandey A., Roussos S. (eds) Coffee Biotechnology and Quality. Springer, Dordrecht.

https://doi.org/10.1007/978-94-017-1068-8_45.

Nadir, A.H. (2014). Effect of different substrates on the yield and quality of oyster mushroom

(Pleurotus ostreatus). European University of Lefke Institute of Graduate Studies and Research, 1-48.

Nasrollahi, Z., & Yadegari, M. H. (2016). Antifungal Activity of Caffeine in Combination with

Fluconazole against Candida albicans. Infect Epidemiol Med., 2(2), 18–21.

https://doi.org/10.18869/modares.iem.2.2.18

Nirosha, A., Rajeshkanna, S. & Mikunthan, G. (2018) Effect of oil cakes and garlic aqueous based

formulations of Trichoderma viride on management of meloidogyne incognita in chilli. Open

Agriculture, Vol. 3 (Issue 1), 214-219. https://doi.org/10.1515/opag-2018-0022

Pervez, Z., Islam, M. S., & Islam, S. M. A. (2017). Evaluation of some plant extracts in controlling

green mold (Trichoderma harzianum) associated with substrate of oyster mushroom. Bangladesh

Research Publication Journal, 7(3), 194–200.

Rakatama, A., Pramono, A. and Yulianti, R. (2018). The Antifungal Inhibitory Concentration

Effectiveness Test From Ethanol Seed Arabica Coffee (Coffea arabica) Extract Against The Growth

Of Candida albicans Patient Isolate With In Vitro Method. Journal of Physics: Conference Series,

Sugiyama, A., Cecile M., Sano, H. & Yazaki, K. (2016) Caffeine fostering of mycoparasitic fungi

against phytopathogens. Plant Signaling & Behavior, 11:1, DOI: 10.1080/15592324.2015.1113362

Yang, D., Liang, J., Wang, Y., Sun, F., Tao, H., Xu, Q., Zhang, L., Zhang, Z., Ho, C.T. & Wan, X.

(2016) Tea waste: an effective and economic substrate for oyster mushroom cultivation. J Sci Food

Agric 96(2):680–684.

Yang, W., Guo, F. & Wan, Z. (2013). Yield and size of oyster mushroom grown on rice / wheat straw

basal substrate supplemented with cotton seed hull. Saudi Journal of Biological Sciences, 20(4), 333–

https://doi.org/10.1016/j.sjbs.2013.02.006

Published
2021-08-20
How to Cite
Pakeerthan, K. (2021). ECO-FRIENDLY MANAGEMENT COMMON LAB CONTAMINANT Trichoderma spp IN OYSTER MUSHROOM PRODUCTION USING AGROBASED INDUSTRY’S BY-PRODUCTS. Proceedings of The International Conference on Climate Change, 5(1), 65-71. https://doi.org/10.17501/2513258X.2021.5105