PRODUCTION OF PROTEIN HYDROLYSATES FROM FISH SKIN FOR DAIRY PRODUCTS

Authors

  • E.I. Titov Moscow State University of food production, Russia
  • G.V. Semenov Moscow State University of food production, Russia
  • I.I. Ionova Moscow State University of food production, Russia
  • I.S. Krasnova Moscow State University of food production, Russia
  • N.S. Kozlov Moscow State University of food production, Russia

DOI:

https://doi.org/10.17501/biotech.2018.3103

Keywords:

fish protein hydrolysates, hydrolysis, fish odour, dairy products

Abstract

The aim of this research was studying the fish protein hydrolysates (FPH) produced from Salmon (Salmo salar) skin prepared by different methods. Four methods were used: enzymatic hydrolysis, acid hydrolysis, chemical (with acids or alkalis) and enzymatic hydrolysis with preserving agents upon the heating proposed to produce fish protein hydrolysates (FPH). All methods modified and adapted to optimized researches. All hydrolysates were vacuum freeze-dried and collagen content was determined in them. Depending on the collagen content in the FPH, methods may be ranked as follows: chemical method (with acids or alkali) (15,1%) < enzymatic hydrolysis (26,5%) < acid hydrolysis (30,0%) < enzymatic hydrolysis with preserving agents upon the heating (37,5%). The results showed that FPH prepared using enzymatic hydrolysis with preserving agents upon the heating had a greater amount of protein than FPH prepared using other methods. Citric acid preparation and sesame seeds covered the initial fish odour and off-flavours generated in fish hydrolysates. The sensory analysis of the final product allowed choosing the most rational content of components. Hydrolysis with preserving agents on the heating allowed obtaining collagen hydrolysate in dry form and without fish smell. This FPH may be used as a stabilizer in dairy products.

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References

Bae, I., Osatomi, K., Yoshida, A., Osako, K., Yamaguchi, A. and Hara, K. 2008, Biochemical properties of acid-soluble collagens extracted from the skins of underutilised fishes. Food Chemistry, 108, 49-54.

Bower, J., 2013, Statistical Methods for food Science. (Edinburgh: Wiley-Blackwell).

Dillow A. K. and Lowman A. M., 2002, Biomimetic Materials and Design: Biointerfacial Strategies, Tissue Engineering, and Targeted Drug Delivery (Mercel Dekker, New York, NY, USA).

Hashim, P., Mohd Ridzwan, M. S., Bakar, J. and Mat Hashim, D., 2015, Collagen in food and beverage industries. International Food Research Journal, 22(1), 1-8.

Mintz, G.R., Reinhart, G.M. and Lent, B., 1991, Relationship between collagen hydrolysate molecular weight and peptide substantivity to hair. Journal of the Society of Cosmetic Chemists, 42, 35-44.

Proksch, E., Schunck, M., Zague, V., Degwert, J. and Oesser, S., 2014, Oral intake of specific bioactive collagen peptides reduces skin wrinkles and increases dermal matrix synthesis. Skin Pharmacology and Physiology, 27(3), 113-119.

Ramasamy S., and Ramadhar K., 2015, A Novel Enzymatic Method for Preparation and Characterization of Collagen Film from Swim Bladder of Fish Rohu (Labeo rohita). Food and Nutrition Sciences, 6(15), 1468-1478.

Silva T.H., Moreira-Silva J., Marques A.L.P., Domingues A., Bayon Y. and Reis R. L., 2014, Marine Origin Collagens and Its Potential Applications. Mar. Drugs, 12, 5881-5901.

.Shironina, A.Y. 2015. Improving the Technology of Fish Proteins Proteolysis and the Study of Colloid Chemical Properties of Hydrolysates. (Murmansk, Russia).

Shori, A.B., Baba, A.S., and Chuah, P.F., 2013, The effects of fish collagen on the proteolysis of milk proteins, ACE inhibitory activity and sensory evaluation of plain- and Allium sativum-yogurt. J. Taiwan Inst. Chem. Eng. 9, 1-6

Thorkelsson, G., Slizyte, R., Gildberg, A., Kristinsson, H.G., 2009, Fish proteins and peptide products: processing methods, quality and functional properties, in Marine Functional Food, Edited by J.B. Luten, (Wageningen Academic Publishers), pp.115-140.

Yamamoto, K., Igawa, K., Sugimoto, K., Yoshizawa, Y., Yanagiguchi, K., Ikeda, T., Yamada, S. and Hayashi, Y., 2014, Biological safety of fish (tilapia) collagen. Biomed. Res. Int., 1-9.

Yang S.-F., Leong K.-F., Du Z.-H., and Chua C.-K., 2001, The design of scaffolds for use in tissue engineering - part I. Traditional factors, Tissue Engineering, 7(6), 679–689.

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Published

2019-05-21

How to Cite

Titov, E., Semenov, G., Ionova, I., Krasnova, I., & Kozlov, N. (2019). PRODUCTION OF PROTEIN HYDROLYSATES FROM FISH SKIN FOR DAIRY PRODUCTS. Proceedings of International Conference on BioScience and Biotechnology, 3(1), 17–27. https://doi.org/10.17501/biotech.2018.3103